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Evi Timotheou | BSc, MSc

Senior Clinical Embryologist, Lab Director


Evi Timotheou received her Bachelor of Science degree in Biology from University of Bologna, Italy in 2004. Her thesis included research about the influence of different culture systems in human ovarian tissue preservation after cryopreservation.

Evi  began her embryology career in 2004 at University Hospital Sant’ Orsola Malpighi, Institute of Gynecology & Obstetrics, Human Reproduction Unit, Bologna, Italy , trained by Raffaella Fabbri  in freezing / thawing ovarian tissue , ovarian tissue culture and electron microscopy techniques.

She completed her internship in IVF techniques at Ravenna Hospital, Department of Obstetrics & Gynecology, Italy in 2006.

In 2007 she was recruited as embryologist at Genesis IVF Center, Patras, Greece.

She joined American Fertility Services Clinic in New York, USA in 2007 and specialized in assisted reproduction techniques including pre-implantation genetic diagnosis (PGD) and extended embryo culture.


In 2009 Evi joined the Team of embryologists at “IVF Athens Center” in Athens, Greece. She obtained her MSc. in Reproductive Science from University of Athens, Medical School. Her research included use of metabolomics in in-vitro fertilization for the embryo development and their relation with the genetic profile of women.

From 2013 Evi is ESHRE certified Clinical Embryologist.

She joined Assisting Nature as a Senior Embryologist in 2015 and was promoted to Laboratory Director in January 2016. Evi is responsible for all functions of the clinical laboratory including in vitro fertilization, ICSI, assisted hatching, testicular and epididymal sperm preparation, preimplantation genetic diagnosis, oocytes and embryo cryopreservation. Finally she is responsible for the quality assurance and quality control of the Embryology Laboratory. 

She has co-authored numerous research journal articles in her field and is an active member of The European Society of Human Reproduction and Embryology.

Email: [email protected]

Scientific publications, presentations, papers

  1. Papanikolaou E, Timotheou E, Tatsi P, Chartomatsidou T, Asouchidou I, Grynberg M, Vlachou C, Katsoula E, Najdecki R. Long-Antagonist protocol versus Classical-day-6 Antagonist. Single luteal use of long-acting GnRH-antagonist Degarelix can efficiently downregulate LH during ovarian stimulation for IVF achieving comparable pregnancy outcome. ESHRE Annual Meeting, Vienna 2019 (oral presentation)
  2. Najdecki R, Choulaira F, Timotheou E, Tatsi P, Chartomatsidou T, Asouchidou E, Pakaki F, Bouchlariotou S, Humaidan P, Papanikolaou E. Single follicular degarelix, a new long acting GnRH-antagonist for LH surge suppression during ovarian stimulation in oocyte donors. A randomised controlled trial. ESHRE Annual Meeting, Vienna 2019 (oral presentation)
  3. Timotheou E, Chartomatsidou T, Tatsi P, Katsoula E, Vlachou C, Stamataki S, Asouchidou I, Zafeiratis O, Najdecki R, Papanikolaou E. Cumulative Live Birth Rates (CLBR) in patients following Freeze-all policy are higher compared to patients who undergo fresh embryotransfer (ET) followed by frozen-thawed embryotransfers. ESHRE Annual Meeting, Vienna 2019 (poster presentation)
  4. Papanikolaou E, Chartomatsidou T, Timotheou E, Tatsi P, Katsoula E, Vlachou C, Asouchidou I, Zafeiratis O, Najdecki R. In Freeze-All Strategy, Cumulative Live Birth Rate (CLBR) Is Increasing According to the Number of Blastocysts Formed in Women <40 Undergoing Intracytoplasmic Sperm Injection (ICSI). Front Endocrinol. 2019; 10 (427) https://www.frontiersin.org/articles/10.3389/fendo.2019.00427/full
  5. Papanikolaou EG, Timotheou E, Tatsi P, Janssens H, Grynberg M, Athanasiadis A, Zafeirati C, Najdecki R, Petousis S. COMFFETI, Combined Fresh and Frozen Embryo Transfers per Indvidual: A New Index of Quality Control for the Performance of Embryologic Labs in The Emerging Era of Segmentation of Cycle and Freeze- All Strategy. Int J Fertil Steril. 2019; 12(4): 339-342 https://www.ncbi.nlm.nih.gov/pubmed/30291697
  6. Papanikolaou EG, Yarali H, Timotheou E, Grynberg M, Zafeiratis O, Tournaye H and Najdecki R. A Proof-of-Concept Clinical Trial of A Single Luteal Use of Long-Acting Gonadotropin-Releasing Hormone Antagonist Degarelix in Controlled Ovarian Stimulation for In Vitro Fertilization: Long Antagonist Protocol. Front. Endocrine. 2018. 9:25. doi: 10.3389/fendo.2018.00025     https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5839166/
  7. Papanikolaou EG, Yarali H, Timotheou E, Tatsi, P, Chartomatsidou T, Grynberg M, Zafeiratis O, Vlachou C, Tournaye H  and Najdecki R. Long-Antagonist protocol; single luteal use of long-acting GnRH-antagonist Degarelix can efficiently downregulate LH during ovarian stimulation for IVF. A proof of concept. ESHRE Annual Meeting, Barcelona 2018 (Award nominee)
  8. Chartomatsidou T, Timotheou E, Tatsi P, Katsoula E, Zafeiratis O, Najdecki R, Papanikolaou E. In Freeze-all strategy Cumulative Live Birth Rate (CLBR) is increasing according to the number of blastocysts formed in women <40 undergoing Intracytoplasmic Sperm Injection (ICSI). ESHRE Annual Meeting, Barcelona 2018
  9. Papanikolaou E, Timotheou E, Tatsi P, Chartomatsidou T, Najdecki R. Long-Antagonist protocol; luteal administration of a single dose of Degarelix (long-acting GnRH-antagonist) for controlled ovarian stimulation. A new protocol for IVF. ASRM Scientific Congress & Expo, Denver, Colorado, 2018
  10. The 20th World Congress on Controversies in Obstetrics, Gynecology & Infertility (COGI) Paris 2014: oral presentation
  11. Influence of protein supports on ovarian tissue cryopreservation”. 7th World Congress on Controversies in Obstetrics Gynecology & Infertility, Athens. R. Fabbri, S. Di Tullio, G Bracone, E. Timotheou, G. Pasquinelli, B. Mozzanega, S. Venturoli.
  12. «1st National Workshop Theorical-Practical of Human Ovarian Tissue Cryopreservation», S. Orsola-Malpighi Hospital, Bologna, Italy (speaker & supervisor)
  13. Clinical and Molecular description of Prenatal Diagnosis fetal micro size 2.5 Mb duplication in the region 22q11.2 maternal origin”.Christopoulou G., Sismani K., Sakellariou Μ., Timotheou Ε., Magkana Μ., Koutsouni Α., Kostomenos D., Ntoutsoulis G., Velissariou V., Αthanassiou V.
  14. Birth of healthy baby after Preimplantation Genetic Diagnosis and Vitrification of a Woman with Achondroplasia. 17th National Congress of Perinatal Medicine. Athens. Sakellariou Μ., Bili C., Florentin L., Timotheou Ε., Magkana Μ., Koutsouni Α., Pitta P., Gouedard C., Kostomenos D., Ntoutsoulis G., Αthanassiou V.
  15. “Γέννηση υγιούς παιδιού μετά από Υαλοποίηση ωαρίων και Κρυοσυντηρημένο Ορχικό Ιστό: CaseReport. 12ο Πανελλήνιο Συνέδριο Μαιευτικής- Γυναικολογίας. Thessaloniki. Μ. Sakellariou, Ε. Timotheou, Μ. Magkana, Α.Κ. Koutsouni, G. Nikolopoulos, G. Ntoutsoulis, D. Kostomenos, V. Athanassiou.
  16. The impact of zona pellucida thickness on day-5 blastocysts in clinical. Vasilios Athanassiou, Evangelia Timotheou, Georgios Ntoutsoulis, Dimitrios Kostomenos, Georgios Nikolopoulos, Maria Sakellariou.
  17. Prolonged warming period improves clinical pregnancy rate of early day-5 vitrified blastocyst. Vasilios Athanassiou, Maria Sakellariou, Georgios Nikolopoulos, Dimitrios Kostomenos, Georgios Ntoutsoulis, Evangelia Timotheou.