Decreasing embryo stress is a key factor in long term incubation. The G210 has several characteristics to ensure minimal stress.
Optimal temperature conditions at all times. The advanced heating system of the G210 ensures constant and all-embracing heating in the chambers
Embryo conditions including pH levels are kept stabile through the combination of accurate gas and temperature control.
In case any of the values exceeds the pre-defined normal range, will rise visual and audible alarms and send a text message to the lab staff in order to handle the situation.
Ensuring the maintenance of the ideal conditions in an embryo culture, is of a great importance, as they are of the most crucial factors that determine further survival and development. These conditions are maintained in special incubators that allow continuous control and stability of all the important parameters, such as temperature, pH, humidity and concentrations of O2 and CO2 in the embryo atmosphere. Despite the importance of maintaining these parameters stable, during the whole time of the embryo culture, at some point, embryos have to assessed under the microscope. This has to be done on day 1, in order to check the fertilization rate and during the 3rd and/ or the 5th day, to ensure that the embryos are developing properly. Of course, in order to observe the embryos, they must leave the comforting conditions of the incubator.
Despite, the fact that the duration that the embryos leave the incubator is limited to the minimum, the ideal conditions of embryo culture are disrupted. Recently, the evolution of time lapse incubators led to a new, novel system in embryo culture. A time lapse incubator contains an intergraded video system that allow the continuous digital imaging of the embryos. In this way, embryo cultures can be assessed in a detailed and constant manner, since the fertilization day and until the time of the embryo transfer. Of course, time lapse incubators offer the advantage of a substantial limitation in embryo handling and condition disruption. In addition, this kind of incubators allow the imaging of important embryonic developmental stages, such as the appearance of the pronuclei and the cleavage/ blastocyst stage. Moreover, time lapse incubators offer the embryologists new morphokinetic values of embryo assessment, to optimize selection of embryos that can lead to pregnancy.