The first step for sperm cryopreservation is the collection of the sample. Sperm is collected in a special room (men’s room) next to the laboratory in the clinic and then delivered to the Laboratory staff. The embryologists evaluate the concentration and motility of the sperm sample and then the cryopreservation procedure begins. A special cryoprotectant is added to the sample that protects the spermatozoa from the low temperature and then a gradual decrease of temperature is happening until finally the sample is stored at liquid nitrogen at -196ο C in special storage tanks.
Testicular Tissue Cryopreservation
In cases of azoospermia, testicular biopsy is a very common procedure in order to examine if the testis produce spermatozoa or not. Testicular tissue is obtained and after special preparation the embryologists examine the tissue for mature spermatozoa under the reverse microscope. If spermatozoa are found, the sample is cryopreserved and stored for future use in an IVF program.
Sperm and Testicular Tissue Cryopreservation is recommended and applied successfully for many years. Only in cases with exceptionally low sperm sample concentration is not beneficial and may not be recommended.